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    Genetic relationships between mtDNA control region sequences bp from dingoes, domestic dogs, and wolves.

    A Phylogenetic tree showing all dog, wolf, and dingo mtDNA types.

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    Unlabeled leaves denote mtDNA types from domestic dogs, yellow circles denote types unique to dingoes, red circles denote types found in both dingo and dog, and squares denote wolf mtDNA types. The tree is rooted to coyote sequences.

    Dog clades A—F are indicated with letters. B Minimum-spanning network of the main dog clade clade A. Sequence types circles and empty nodes solid dots are separated by one mutational step substitutions; indels are not shown. The mtDNA type indicated by bold lines has four shortest links with a length of three mutational steps to other mtDNA types, but two of these are not shown to simplify the figure. Yellow, unique dingo mtDNA type; red, type found in both dingo and dog; blue, type found in dogs in Indonesia, the Philippines, or Malaysia; green, unique New Guinea type.

    Calculation of Genetic Distances. Based on the assumption that A29 was the only founder mtDNA type, an estimate of the time for the introduction of dingoes to Australia was calculated from the mean distance to A29 among dingo sequences.

    The mutation rate of the analyzed region was estimated from the mean genetic distance between the dingo, dog, and wolf mtDNA types and the coyote types in the phylogenetic tree Fig. Results mtDNA was analyzed in Australian dingoes sampled in all states of Australia Table 1dogs from all continents, and 38 Eurasian wolves, and bp were analyzed in 19 pre-European archaeological dog samples from Polynesia. The sequence variation among the dingoes was very restricted compared with that of dogs and wolves Fig.

    Among the dingoes there were 20 mtDNA types differing by at most two substitutions, whereas among dogs there were mtDNA types with a maximum difference of 16 substitutions between mtDNA types. It has been shown that in a phylogenetic tree of dog and wolf sequences, domestic dog mtDNA types form several separate branches distributed among the wolf mtDNA types, showing that the domestic dog originates from several maternal wolf lines 10 A minimum-spanning network of clade A shows the dingo mtDNA types forming a single internal cluster around a central type, A29, found in both dingoes and dogs Fig.

    Interestingly, for the region sequenced for both ancient and contemporary samples, Arc2 is identical to mtDNA type A75, which was found only in two Indonesian samples, whereas Arc1 has a sequence found in a number of widely spread types The mutation rate for the analyzed region was estimated from the mean genetic distance between the dingo, dog, and wolf mtDNA types and the coyote types in the phylogenetic tree Fig.

    The star-like formation of the dingo mtDNA sequences in the minimum-spanning network Fig. Assuming that A29 was the only founder type, the probable time for the introduction of dingoes to Australia was estimated at 4,—5, yr ago from the mean distance to A29 among dingo sequences 0. Taking into account the possibility of a split up to 2 Myr ago, the introduction would have happened some time 4,—10, yr ago.

    The mean distance to A29 among dingo sequences differs considerably between Western Australia and the other parts of Australia Table 1. This discrepancy is likely caused by random genetic drift in the Pilbara District population from which most Western Australian samples 25 of 29 were taken, and therefore the mean value for all dingo samples was used. Discussion The position of the dingo mtDNA types in the middle of the dog mtDNA variation, A29 being found among both dogs and dingoes and forming an internal node in clade A surrounded by the other dingo mtDNA types as well as by dog mtDNA types, provides strong evidence that dingoes originated from domesticated dogs.

    It was shown before that domestic dogs in Eurasia, America, and Africa have a common origin 1120and with this study it is shown that this applies to domesticated dogs on all continents. The minimum-spanning network of phylogenetic clade A has a complicated pattern of dog haplotypes, caused by a large degree of homoplasy, which shows that the analyzed region is not sufficient for obtaining a phylogenetic resolution of dog mtDNA types.

    However, the dingo types are well grouped, and the very restricted sequence variation among dingoes, with more than half of the dingoes having mtDNA type A29 and all other mtDNA types forming a star-like formation around A29, and the fact that all other dingo types but A9 were unique to dingoes suggest that all dingo mtDNA types originate from A This indicates that the dingo population was formed either from very few dogs, theoretically as few as a single pregnant female, or from a group of dogs that had radically lost genetic variation through one or several severe bottlenecks on their way from the Asian mainland through Island Southeast Asia.

    However, the presence of a number of mtDNA types other than A29 on the islands surrounding Australia, both in modern samples and in samples from pre-European archaeological sites, indicates that there were several mtDNA types other than A29 present in the region and points at a single founding event.

    It also indicates that there have not been any substantial later introductions of dogs to Australia before the arrival of Europeans. However, because of the maternal mode of inheritance of mtDNA, hybridization between male dogs and female dingoes would not be identified by these mtDNA analyses.

    It can be noted that, similar to dingoes, the New Guinean dogs had the mtDNA type A29 and a unique type differing from A29 by one substitution. These so-called New Guinea singing dogs are feral and show some morphological and behavioral similarities to dingoes. A common origin and some gene flow between the two populations is therefore possible. Among domestic dogs, A29 was found only among East Asian, Island Southeast Asian, and American dogs, and the mtDNA types radiating from A29 in the minimum-spanning network were found almost exclusively in East Asia 11strongly indicating an East Asian rather than Indian origin for the dingo ancestor.

    In conclusion, this study of mtDNA sequence variation among dingoes provides a number of clues from which a detailed picture of the origin and history of the Australian dingo can be derived. The dingo originated from a population of East Asian dogs. The dingo population was probably founded from a small number of animals, as the last trickle of domestic dogs through a series of bottlenecks, or even by a single chance event and has since remained effectively isolated from other dog populations.

    By this time, domestic dogs had existed for several thousand years 411and the present semidomestic state of the dingo can probably be attributed to a long existence as a feral animal.

    The sequences reported in this paper have been deposited in the GenBank database accession nos. Van Nostrand Reinhold, New Yorkpp. Hesperian, Carlisle, WApp. Its Evolution, Behaviour and Interactions with People, ed.
    Peter Wang Leave a Comment When travel to Australia, you will be surprised by how many attractive women in Sydney, Melbourne, Brisbane and other cities in the country.

    Australia is one of the best places in the world to find girls for friendship and marriage. With proper social and romantic skills you can find your best matches in all our major cities. Today with the boom of online dating, you can find Australian girls through many online dating services. Here is list of the best Australian dating sites for singles. But there are quite a few single women on Aussie Cupid from outside of Australia. Some are looking for committed relationship while there are many people who are just for casual hookups.

    I advise you to use video chat to identify if the person you are talking is a real person instead of a scammer. Remember that in order to protect your privacy do not reveal your real email address. Aussie Cupid has a cool language translation tool, it can automatically translate the email for you. You can send interest to other members and talk to Gold and Platinum members.

    But you can not talk to a standard member free memberyou can only talk when at least one of you is a paid member. If you like Australian girls, you should go to Aussie Cupid.

    It does not have as big pull as big dating sites like POF or RSVP, but it works well for those who are flexible in who they meet and where they live, you have bigger chance to have an international relationship there. It has about 2 million members in total. But you need to pay when you initiate a talk with someone you like by email or chat. RSVP is mainly designed for people in Australia. It is the best dating site if you are a single in Australia.

    But if you are from regions outside of Australia, you should consider Aussie Cupid. Sign Up For Free! It has more than one million Australian members and most are at their 20s and 30s.

    It is free to sign up and free members enjoy limited functions like creating profile, view daily matches and limited communication with the matches.

    Paying members can see who are interested in them and check the photos of their matches or even phone their matches. Besides guys and girls from Australia, you can also find many members from other regions of the world such as Latin singles.

    There are about 1. It is almost completely free. You can use its advanced matching system to find your love. Members can chat by either audio or video. OkCupid OKCupid is a popular dating site in the world and it has a decent number of Australian members. You will know this is true when check members of singles community of Sydney or Melbourne.

    It is free to register and free members can browse profiles and photos and contact others but there is a cap on it. Only paying members can check the full list of people who like them and other make profiles more attractive. Users can contact other members via messages or live chat. OkCupid is one of the best Australian dating sites that you should trust, give it a try and you will know it is a good choice for you. Plenty of Fish has two levels of memberships. Keep in mind that subscriptions automatically renew if you do not quit the plan.

    As it is free to register, you may find that you may get low reply rate. Many users use the site just for fun and then leave quickly. Visit Plenty of Fish 7. The most Tinder users are young people aged from There are no charges for this app, users need to sign up with Facebook account, which makes sure that the members are real people.

    Tinder use your mobile phone to discover nearby users and help you find other members in you local area. Most Tinder users are young people who hope to know each other or just for casual sex, only a small part of members are looking for serious relationships. Zoosk Zoosk is a popular dating site in the world and it also has a large membership base in Australia.

    Most of its members aged in their late 20s and early 30s. It is free to sign up and users enjoy basic functions such as creating a profile, searching for singles, and sending messages. Paid membership allows to send and receive messages, check who have viewed your profiles and use their own matching service.

    Zoosk also has mobile dating apps for both Android and iOS platforms. Above are the reviews of best dating sites in Australia. Try these dating sites and find your Australian love right now, please let me know what you think of these sites and leave your comments below.
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